CD Skripsi

Efek Human Platelet Lysate Terhadap Kapasitas Migrasi Sel Punca Mesenkimal Tali Pusat

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Mesenchymal stem cells (MSC) are multipotent stem cells with proliferation, differentiation, and immunomodulatory capabilities that are potential for therapy of various diseases. The migration capacity of MSC is very important in the success of MSC therapy. The aim of this study was to compare the effect of human platelet lysate (HPL) and fetal bovine serum (FBS) supplementation on MSC migration capacity. The design of this study was laboratory experimental research. Mesenchymal stem cells isolated from umbilical cord using explant method were then cultured into 24-well culture plates with 5% HPL, 10% HPL, and 10% FBS supplementation (control) for 24 and 48 hours. Migration capacity was assessed using the scratch assay method and then processed using ImageJ software. One-way ANNOVA and Kruskal-Wallis tests were used to analyze differences in MSC migration capacity within 24 and 48 hours and Two-way ANNOVA test to analyze the interaction between HPL concentration and exposure time. The results showed that there was no significant difference in the degree of closure in scratch gap of HPL and FBS supplemented MSC for 24 hours (p = 0.88) and 48 hours (p = 0.27). In addition, this study found no interaction between HPL concentration and exposure time in affecting the closure of MSC scratch gaps (p = 0.28). In conclusion, the migration capacity of umbilical cord MSC supplemented with HPL is insignificantly different compared to MSC supplemented with FBS, thus HPL has potential as an alternative to FBS for MSC culture supplementation.

Keywords : human platelet lysate, fetal bovine serum, migration capacity, scratch assay

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2108111407

Penerbit

Pekanbaru : Universitas Riau FK Pendidikan Dokter., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2108111407

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PENDIDIKAN DOKTER

Info Detail Spesifik

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Pernyataan Tanggungjawab

Jaka

Versi lain/terkait

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