Image of Profil Senyawa Antimikroba Fermentasi Ko-Kultur Aspergillus Fumigatus Lbkurcc269 Dan Bacillus Paramycoides Lbkurcc218 Melalui Pendekatan Metabolomik =Antimicrobial Compound Profiling From Co-Culture Fermentation Of Aspergillus Fumigatus Lbkurcc269 And Bacillus Paramycoides Lbkurcc218 Using A Metabolomics Approach
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Profil Senyawa Antimikroba Fermentasi Ko-Kultur Aspergillus Fumigatus Lbkurcc269 Dan Bacillus Paramycoides Lbkurcc218 Melalui Pendekatan Metabolomik =Antimicrobial Compound Profiling From Co-Culture Fermentation Of Aspergillus Fumigatus Lbkurcc269 And Bacillus Paramycoides Lbkurcc218 Using A Metabolomics Approach

The discovery of new antimicrobial compounds is critical due to increasing resistance of microorganisms to existing antimicrobials. Thermophilic fungi and bacteria from extreme environments, such as hot springs, show potential as sources of bioactive compounds due to their unique metabolic adaptations to high temperatures. In this study, bioactive compounds from Aspergillus fumigatus LBKURCC269, Bacillus paramycoides LBKURCC218, and their co-culture were analyzed using metabolomic approaches involving GC-MS and LC-MS/MS. Bioactive secondary metabolites were extracted using ethyl acetate and tested against Candida albicans, Escherichia coli, and Staphylococcus aureus. The co- culture demonstrated higher antimicrobial activity than the monocultures. GC-MS analysis revealed that the ethyl acetate extracts of A. fumigatus and B. paramycoides monocultures produced 24 and 33 compounds, respectively, while the co-culture yielded 42 compounds, 20 of which were unique to the co-culture. The main bioactive metabolites identified as antimicrobial in the co-culture fermentation extracts were pentadecanoic acid, cyclopropane pentanoic acid and 3-piperidinol. LC-MS/MS analysis using multivariate analysis and the Global Natural Products Social (GNPS) platform provided a molecular network that revealed additional antimicrobial compounds such as 2-(-4-hydroxybenzyl)-4-(3- acetyl) quinazolin-one and novel pyrazine metabolites N7 and N8, which are putative 3-(propan-2-yl)octahydroxypyrrolo[1,2-a]pyrazine-1,4-dione derivatives. Fermentation conditions for co-culture antimicrobial production were optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM). Optimal conditions 60.5°C, pH 8.68, and 0.329 g/L NaCl resulted in a twofold increase in antimicrobial activity compared to unoptimized conditions. These findings highlight the potential of metabolomic approaches and co-culture fermentation in the discovery and production of novel antimicrobial compounds.



Key words: Antimicrobial, Co-culture fermentation, Metabolomics approach, Thermophilic microbes.

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Perpustakaan Universitas Riau 1810346989
1810346989
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1810346989
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Pekanbaru : Universitas Riau PASCASARJANA S3 Ilmu Kimia.,
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Indonesia
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1810346989
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DISERTASI S3 ILMU KIMIA
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