CD Skripsi

Deteksi Polutan Air Berbahan Aktif Atrazine Menggunakan Mikrobial Biosensor Alga Chlorella Sp.

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Water pollution caused by agricultural and industrial activities has become a major environmental concern in recent years, as it degrades water quality and threatens the balance of aquatic ecosystems. One of the primary sources of water pollution is the use of Atrazine-based herbicides, which have a long half-life in aquatic environments and potentially disrupt aquatic ecosystems. Therefore, regular water quality monitoring is essential as a preventive measure. One of the key parameters used in water quality assessment is dissolved oxygen (DO), which serves as an indicator of pollution levels. This study aims to detect water contamination caused by Atrazine-based herbicides using an amperometric microbial biosensor with Chlorella sp algae. As the bioreceptor. Chlorella sp. was chosen for its photosynthetic ability, which produces dissolved oxygen as an indicator of water quality. The experiment was conducted with varying Atrazine concentrations of 10 mg/L, 50 mg/L, 100 mg/L, 200 mg/L, 300 mg/L, and 500 mg/L, under red LED light exposure with a wavelength of 620-630 nm. Dissolved oxygen and pH levels were measured after Atrazine exposure to evaluate its impact on algae photosynthetic activity. The measurement results indicated that DO levels decreased with increasing Atrazine concentration, accompanied by a proportional decrease in pH. The recorded DO values for each Atrazine concentrations were 112.21%; 106.99; 99.20%; 82.92%; 73.45%; and 56.64%, while the corresponding pH values were 6.4; 5.84; 4.92; 4.16; 3.47; and 2.13. Based on the measurements, the sensor electrode demonstrated high sensitivity in detecting small changes in Atrazine concentration, with a sensitivity of 0.26 mV/mg.L-1Atrazine, a Limit of Detection (LoD) of 3.61 mg/L, and a Limit of Quantification (LoQ) of 12.04 mg/L. Stability testing revealed that the algae maintained 92% of its initial activity after 7 days of storage. Data clustering and visualization using Principal Component Analysis (PCA) indicated that the biosensor effectively distinguished Atrazine concentrations with a total variation of 87.6%. These experimental findings demonstrate that the amperometric biosensor based on Chlorella sp. can be effectively applied for detecting Atrazine-based herbicides and exhibits strong biosensor characteristics, making it a promising tool for water quality monitoring.

Keywords: Amperometric Biosensor, Chlorella sp., Atrazine, Dissolved Oxygen, LED Light, Principal Component Analysis
 

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2003110344

Penerbit

Pekanbaru : Universitas Riau FMIPA Fisika., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2003110344

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

FISIKA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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