CD Skripsi

Isolasi Escherichia Coli Dari Daging Sapi Dan Karakterisasi Genotipnya Menggunakan Metode Random Amplified Polymorphic Dna

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Beef is potential as a medium for microbial growth, such as coliform bacteria, since it has a high nutrient content. Coliform bacteria in a given amount could be used as a higyene indicator and a sign for the presence of pathogenic bacteria. One species of coliform bacteria that often contaminate meat is Escherichia coli. E. coli is a normal flora of the gastrointestinal tract, some strains of them are pathogens such as E. coli O157:H7 that cause diarrhea, hemorrhagic colitis, hemolytic uremic syndrome, and even leads to death in humans. The aim of this study was to calculate the number of coliform bacteria, to detect the presence of E. coli in beef sold in traditional and modern markets at Pekanbaru using the Most Probable Number method, to detect the presence of E. coli O157:H7 with Most Probable Number-Polymerase Chain Reaction (MPN-PCR) methods, and to characterize the E. coli isolated from all samples studied using the Random Amplified Polymorphic DNA method. The results showed that all samples were found to be contaminated by E. coli and coliform bacteria. The number of coliform bacteria in all samples exceeded the maximum threshold of bacterial contamination according to Indonesian National Standard No. 7388:2009. There was not any sample contaminated by E. coli O157:H7. RAPD analysis separated the E. coli isolates into two main clusters which highest level of genetic similarity was 98% for the isolates of EC17 and EC18 sold at Arengka Market.
Keywords: Coliform, E. coli, MPN, PCR, RAPD.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02.114 (0066)

Penerbit

Pekanbaru : Universitas Riau - FMIPA – Kima., 2014

Deskripsi Fisik

xv, 51 hlm.;ill.; 29 cm

Bahasa

ISBN/ISSN

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Klasifikasi

03 02.114 (0066)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

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Pernyataan Tanggungjawab

Dewa

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