CD Skripsi
Kultivasi Mikroalga Menggunakan Media Af6 Berdasarkan Perbedaan Volume Solution A Media Af6
Microalgae are microscopic plant organisms that live in waters. Nutrition is one of the important factors for the growth of microalgae. Optimization of nutrition is expected to increase the growth and production of microalgae lipids. In cultivating laboratory scale microalgae, AF6 media is used as a source of nutrition. One stock of AF6 media material is solution A contains lots of nitrogen. This research purpose for determining the effect of the difference in volume of AF6 media solution A on cell density (optical density), growth rate and percentage of lipids (% lipids) of various microalgae cultivated in AF6 media. In this research observations of cell density microalgae Chlorella sp. (6) and Chlamydomonas sp. (4, 5, 19, chl) on the volume of solution A media AF6 2, 3 and 4 ml were carried out by UV-VIS spectrophotometer with a wavelength of 680 nm while the percentage of microalgae lipids was obtained using the Bligh and Dyer method. The results showed that optical density, specific growth rate highest and shortest doubling time occurs on the addition of a volume of 4 ml of solution A AF6 media. While the highest of %lipids occurred in the volume of 2 ml of the AF6 media solution. Microalgae that have an optical density, specific growth rate, highest lipid content and shortest doubling time are Chlamydomonas sp. Microalgae. (19) 1.3788, 0.91 / day, 28.29% and 0.75 / day, respectively. Therefore, microalgae Chlamydomonas sp. (19) can be cultivated on a commercial scale to be processed into the products we want.
Keywords: lipids, microalgae, AF6 media, optical density, solution A
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