CD Disertasi

Optimalisasi Produksi, Penentuan Struktur Dan Uji Bioaktivitas Senyawa Metabolit Sekunder Jamur Endofit Sporothrix Sp Strain Lokal

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This study aims to find natural, effective and non-resistant sources of new anti-microbial drugs by analyzing ecological, economic and social aspects of the environment. Fungal infections can be caused by environmental factors and bad hygiene such as Candida albicans which causes several diseases, namely oral infections and vaginal discharge in women. Many treatments for diseases caused by C. albicans are unsuccessful due to the occurrence of resistancy and of hepatotoxicity. The use of antimicrobials that do not follow the rules can resistancy. This antimicrobial resistance requires the discovery of more effective new drugs with low toxicity. One of the efforts in fulfilling drug needs and antisipasing drug resistance is by finding and producing new drugs from various sources both plants and microbes which are the background of this dissertation research. There are four components of the study in this study, namely: optimization of production of secondary metabolites of endophytic fungi Sporothrix sp (LBKURCC 43), production, purification and elucidation of the structure of secondary metabolites of endophytic fungus extract Sporothrix sp (LBKURCC 43), Bioactivity Test of F3 Compound , and environmental Analysis.
Sporothrix sp (LBKURCC 43) is an endophytic fungus that lives on dahlia tubers (Dahlia variabilis) which can produce secondary metabolites which are raw materials for new drugs similar to those produced by their hosts, namely Dahlia tuber. The nature, characteristics and test of bioactivity and their impact on the environment will be presented in this dissertation.

Optimization of production of secondary metabolites from endophytic fungi Sporothrix sp (LBKURCC 43): This study uses a method of looking for optimization of environmental factors from fermentation, media, temperature, aeration, and pH. The media fermentation factor was obtained from the optimum medium to produce secondary metabolites, the media variation of Huang et.al (2007) with Na CMC and ammonium sulfate. The temperature factor obtained the optimum temperature at 37̊̊C. The pH of the fermentation is optimum pH 4. For aeration, the optimal 50vvm aeration is obtained.

Production, purification and elucidation of the structure of secondary metabolites of endophytic fungus extract Sporothrix sp (LBKURCC 43): Production of secondary metabolites by optimizing fermentation factors so that extracts of secondary metabolites are obtained. The secondary metabolite extract obtained was purified by various methods. The pure compounds obtained were structural elucidation with a UV-VIS spectrophotometer, IR and HPLC spectrophotometers. The result of elucidation of the structure in obtaining pure compound F3 with its IUPAC name is 1-oxo-1,2,3,4-tetrahydroisoquinolin-4-yl acetate.

Bioactivity Test of F3 Compound Results of isolation: F3 pure compounds were then tested for isolated compounds against the test of Escherichia coli, Staphylococcus aureus and Candida albicans. This is a gram negative, gram positive and pathogenic fungus. The method used is the Kirby Bauer method, which is a disc method to see the inhibitory power given by pure compounds F3. The results showed that pure compounds F3 can inhibit growth of Escherichia coli, Staphylococcus aureus and Candida albicans. But the biggest inhibition is that of Candida albicans where the positive control used is Ciprofloxacin and negative control is the media itself.

Environmental Analysis: Modern drugs circulating in the world are almost partly derived from active ingredients that are isolated and developed from plants. Exploitation of these plants has been carried out since the existence of civilization in searching for medicinal raw materials and attempts to prove traditional medicines used from ancient times. Prevention of over-exploitation can be done by developing a system for producing medicinal compounds from microbes such as endophytic fungi. Ecological research found a new biotechnology, economically feasible for making vaginal cleansing preparations for women. Socially, the community is expected to maintain the biodiversity of the fungus which has the potential as a source of new drug production.

Conclusion: The optimum condition of Sporothrix sp endophytic fungus was obtained with Sporothrix sp (LBKURCC 43) with Na CMC plus sucrose with a ratio of 3: 1 and peptone as a source of nitric and phosphate, agitation 150 rpm, pH 4, 37̊C temperature, and aeration 50 vvm. The chemical structure elucidation test results from sample F3 by the name 1-oxo-1,2,3,4-tetrahydroisoquinolin-4-yl acetate. The bioactivity test results of active F3 compounds as anti-microbial especially as antifungal Candida albicans were seen from the results of analysis with t-test statistics (p

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 01. 319 (0013)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Disertasi Ilmu Lingkungan., 2019

Deskripsi Fisik

xix, 106 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 01. 319 (0013)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PASCASARJANA (DISERTASI) ILMU LINGKUNGAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

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