Image of Respons Pembentukan Kalus Daun Pegagan (Centella Asiatica (L.) Urb.) Dengan Penambahan Naphtalene Acetic Acid (Naa) Dan Benzyl Amino Purin (Bap) Secara In Vitro
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Respons Pembentukan Kalus Daun Pegagan (Centella Asiatica (L.) Urb.) Dengan Penambahan Naphtalene Acetic Acid (Naa) Dan Benzyl Amino Purin (Bap) Secara In Vitro

Gotu kola (Centella asiatica (L.) Urb.) is a medicinal plant that contains chemical compounds as triterpenoids and saponins. The chemical compounds can be produced quickly using in vitro callus induction. The callus is a very important source of planting material in regenerating new plants. Therefore, by inducing callus the need for seedling in large quantities achievable in a short time. This study aimed to determine the effect of the BAP single and combination of BAP and NAA, and determine the effective concentration of a BAP single and combination of BAP and NAA on callus induction of gotu kola leaf explant using in vitro method. This study used Complete Randomized Design (CRD) which consisted of nine treatments (control, 1 mg/l BAP, 2 mg/l BAP, 1 mg/l BAP + 0,1 mg/l NAA, 2 mg/l BAP + 0,1 mg/l NAA, 1 mg/l BAP + 0,3 mg/l NAA, 2 mg/l BAP + 0,3 mg/l NAA, 1 mg/l BAP + 0,5 mg/l NAA, 2 mg/l BAP + 0,5 mg/l NAA) with five replication for each treatment. Data obtained from observation were analyzed descriptively. The results of this research showed that a single BAP and combine of BAP and NAA with a concentration of 1 mg/l BAP is able to provide a callus response in gotu kola leaf explant, where a concentration of 2 mg/l BAP does not form a callus. The best concentration that from the most optimal callus and the highest callus growth (+++) is the treatment of P5 ( 1 mg/l BAP + 0,3 mg/l NAA) which is equal to 100% with the caracteristics of green callus, textured compact and callus covering the entire surface of explants. All the callus produced was textured compact, while the colour of the callus was green and brown.


Keywords : Gotu kola (Centella asiatica (L.) Urb.), Callus, BAP, NAA

Ketersediaan
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Perpustakaan Universitas Riau 03 04.120 (0008)
03 04.120 (0008)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
03 04.120 (0008)
Penerbit
Pekanbaru : Universitas Riau – FMIPA – Biologi.,
Deskripsi Fisik
xiii, 34 hlm.: ill.: 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
03 04.120 (0008)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
BIOLOGI
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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Tidak tersedia versi lain

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