CD Disertasi

Karakteristik Teripang Pasir (Holothuria Scabra J.) Dan Potensi Ekstrak Glukosamin Untuk Produksi Cairan Sinovial Pada Tikus Percobaan

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The skin of sea cucumber (Holothuria scabra), a wasted part, has a potential source of glucosamine for osteoarthritis repair. The purpose of the study was to determine the characteristics of sea cucumber, chitosan extraction and characterization, glucosamine hydrolysis, and its activity in the production of synovial fluid in experimental rats. Materials and Methods: Sea cucumbers, 525+83.6 g in weight were sampled from the Terung Island Waters of Riau Archipelago and Rattus novergicus (Wistar strain), 186+25 g in weight were used for in vivo analysis. Chitin extraction was carried out through the demineralization, decolorization, deproteination; and chitosan was extracted in 50% NaOH solution at 100 °C for 90, 120, 150 minutes. Glucosamine was hydrolyzed in 10%, 15%, 20%, and 25% HCl solution at 120 °C for 15 minutes. Glucosamine was assessed for whiteness degree, yield, water content, LoD, LoI, pH, melting point, solubility, functional group structure test, antioxidant activity, toxicity, and its activity as anti-inflammatory and synovial fluid producing in experimental rats. Results: The skin of sea cucumber consists of dry matter 91.75%, protein 13.21%, fat 0.70%, ash 59.98% and carbohydrates 26.10%. Chitosan extraction for 120 minutes showed the quality value with a yield of 58.44%, whiteness degree 79.41%, solubility rate 92.28%, moisture 6.73%, ash 1.53%, and deacetylation degree 76.83%. Hydrolysis of glucosamine in HCl 15% showed the best parameter values, yield 65.87%, moisture of 5.37%, pH 4.26, LoD 0.85%, LoI 0.39%, melting point 191-194oC, and solubility rate 96.37%, OH stretching group 3292.63 cm-1, NH flexion group 1615.45-1583.63 cm-1, CN 1394.59 cm-1 stretching group, glycosidic group 1139.98-1033.89 cm-1, antioxidant activity (IC50 28.06-42.60 μg/ml) and non-toxic. In vivo tests on Rattus norvegicus showed glucosamine at a dose of 100 mg/kg body weight could reduce the level of inflammation, increase synovial fluid production, and maintain spleen health. Conclusion: Glucosamine of sea cucumber skin had an anti-inflammatory activity and stimulated the synovial fluid production in experimental rats.
Keywords: chitosan, glucosamine, Holothuria scabra, inflammation, synovial

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 03. 320 (0005)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Disertasi Ilmu Kelautan., 2020

Deskripsi Fisik

xxi, 232 hlm,; ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 03. 320 (0005)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

ILMU KELAUTAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

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