CD Skripsi
Aktivitas Antimikroba Bakteriosin Kasar Bakteri Asam Laktat Dari Dadih Terhadap Escherichia Coli Dan Erwinia Carotovora
ABSTRACT
Dadih is one of the West Sumatera’s traditional fermented foods that
contain various strains of lactic acid bacteria. These kinds of LAB could produce
natural preservatives called bacteriocin. This study aimed to determine the
antimicrobial activity of crude bacteriocins from six strains of dadih’s lactic acid
bacteria against Escherichia coli and Erwinia carotovora. The antimicrobial
activity was tested by using the disc paper diffusion method. The results showed
that the crude bacteriocins produced by each LAB had the antimicrobial ability
against Escherichia coli and Erwinia carotovora indicated by a clear zone
between 0,5–9,63 mm. The results also showed that the bacteriocins were
generally resistant at temperature 30 to 100°C, and a pH of 5 to 7, and their
activity decreased slightly below and above this pH range. The highest
antimicrobial activity in inhibiting the growth of E. coli and E. carotovora was
found in the Streptococcus faecalis subsp. liquefaciens R-19 and Streptococcus
faecalis subsp. liquefaciens R-55, respectively.
Keywords: bacteriocins, lactic acid bacteria, antimicrobial activity, dadih,
Escherichia coli, Erwinia carotovora
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