CD Skripsi
Ketahanan Lactobacillus Fermentum Inacc B1295 Yang Dienkapsulasi Dengan Cellulose Microfiber Dari Tandan Kosong Kelapa Sawit Terhadap Kondisi Asam Pada Suhu Dan Waktu Penyimpanan Yang Berbeda
ABSTRACT
Encapsulation aims to protect bacteria from adverse environmental conditions to increase bacterial resistance. The purpose of this study was to measure the resistance of probiotic bacteria encapsulated with cellulose microfiber from oil palm empty bunches to acidic conditions at different temperatures and storage times. This research was carried out experimentally using a factorial, completely randomized design (CRD) consisting of two factors with three replications each. The first factor was the storage temperature which consists of room temperature and 4°C. The second factor was the storage time (0, 7, 14, 21, 28 and 35 days) so that 36 experimental units were obtained. The parameters observed were total lactic acid bacteria, lactic acid bacteria viability and Lactobacillus fermentum InaCC B1295 resistance to acidic conditions. The data obtained were analyzed by Analysis of Variance (ANOVA) then followed by the Duncan Multiple Range Test (DMRT) at the 5% level. The results showed that the storage time and the interaction between the duration and storage temperature had a significant effect (P 0.05) on the percentage of viability and resistance of lactic acid bacteria for 35 days of storage at room temperature and 4°C. This shows that the encapsulation of bacteria with hydrogel CMF from palm oil empty bunches was able to maintain the viability and resistance of bacteria in acidic conditions when stored at room temperature and 4°C for 35 days of storage.
Keywords: Cellulose microfiber, encapsulation, Lactobacillus fermentum
Tidak tersedia versi lain