CD Skripsi

Analisis Sekuens Barkode Dna Pada Daerah Its Dan Trnl- Trnf Intergenic Spacer Pada Tumbuhan Cocor Bebek

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ABSTRACT
Molecular identification can be done through a technique called DNA barcoding
techniques. The noncoding DNA barcodes in plants are ITS and trnL-trnF
intergenic spacer. The ITS region is an area in the nuclear genome that is
commonly used in phylogenetic analysis at the species level, which can show the
highest level of interspecies differences while the trnL-trnF intergenic spacer
region is very suitable for intraspecies phylogenetic analysis. This study aims to
analyze two species of cocor bebek (cocor bebek A and B) growing in Pekanbaru,
Riau. The research methods consisted of morphological observations, sample
preparation, total DNA isolation using the Genomic DNA Mini Kit Plant
(Geneaid), total DNA electrophoresis, PCR using DreamTaq DNA polymerase
(Thermo Scientific), PCR product electrophoresis, sequencing and data analysis
using BioEdit software, BLASTn and MEGA version 6. Morphologically, cocor
bebek A can only be determined up to the genus level, namely Kalanchoe sp. on
the other hand, cocor bebek B can be determined to the species level, namely
Kalanchoe x laetivirens. The length of the ITS DNA sequence and trnL-trnF
intergenic spacer in Kalanchoe sp. of 662 bp and 909 bp, at K. x laetivirens of
684 bp and 908 bp, respectively. In this study, Kalanchoe sp. most similar to the
species Kalanchoe pinnata, and Kalanchoe x laetivirens most similar to the
species Kalanchoe daigremontiana. The number of critical nucleotides in the ITS
and trnL-trnF intergenic spacer sequences were 6 and 13, respectively. The DNA
sequences reported in this study were the first to be reported in Kalanchoe sp. and
K. x laetivirens.
Key words: DNA Barcode, ITS, trnL-trnF Intergenic spacer, Kalanchoe sp.,
Kalanchoe x laetivirens.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 04. 121 (0008)

Penerbit

Pekanbaru : Universitas Riau - Fakultas Matematika Dan Ilmu Pengetahuan Alam - Biologi., 2021

Deskripsi Fisik

ix, 79 hlm.; ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 04. 121 (0008)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

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