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Analisis Keanekaragaman Genetik Gelam (Melaleuca Leucadendra Linn.) Di Pulau Tebing Tinggi Kepulauan Meranti Menggunakan Penanda Random Amplified Polymorphic Dna (Rapd)

Gelam (Melaleuca leucadendra Linn.) plants are potential in wood and non-wood
(essential oil) production. However, it is still underutilized and quite unpopular
particularly compared to essential oil producing cajuputi plants. Gelam naturally
grown in peat forests, therefore this species is suitable for revegetation of peat
land area. The study aimed to analyze genetic diversity of gelam in Tebing Tinggi
Island, Meranti Islands, Riau based on RAPD (Random Amplified Polymorphic
DNA) marker using three arbitary primers. Ten individuals gelam were sampled
in natural population. DNA was isolated from young leaves tissue according to
the manufacturer Genomic DNA Mini Kit Plant (GeneAid) kit protocol. PCR
reaction was carried out under 35 cycle. The results showed that the three RAPD
primers (OPA-04, OPE-03 and OPE-20) generated polymorphism bands with
polymorphism average of 82.3% and PIC value of 0.29 which was informative.
Genetic similarity amongst gelam sample was moderate 0.45-0.86. At the
similarity coefficient of 74%, all samples were divided into four groups. These
result indicated that diversity of gelam population in Tebing Tinggi Island was
considered as moderate category. This gelam germplasm therefore potential for
supporting gelam breeding and peat land revegetation.
Keywords: gelam, genetic diversity, Melaleuca leucadendra Linn., peat, RAPD,
revegetation

Ketersediaan
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Perpustakaan Universitas Riau 03 04.121 (0055)
03 04.121 (0055)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
03 04.121 (0055)
Penerbit
Pekanbaru : Universitas Riau – FMIPA – Biologi.,
Deskripsi Fisik
ix,40 hlm.: ill.: 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
03 04.121 (0055)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
BIOLOGI
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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