CD Skripsi
Uji Resistensi Aedes Aegypti Terhadap Insektisida Organofosfat Melalui Aktifitas Esterase Beta Di Kelurahan Labuh Baru Timur Kota Pekanbaru
Dengue hemorrhagic fever is a dangerous infectious disease in the world. The
increase in dengue cases requires a control effort. DHF vector control that is still
often used today is fogging and larvicides containing organophosphate insecticides.
The use of insecticides for a long time can lead to resistance. This study aims to
determine the resistance of mosquitoes to organophosphate insecticides using
biochemical tests, which measure the activity of non-specific beta esterase enzymes
from Aedes aegypti mosquito larvae in Labuh Baru Timur Urban Village, Payung
Sekaki District, Pekanbaru, Riau, Indonesia. The measurement of esterase activity
using Lee's microplate assay technique is divided into qualitative with visual
identification of color changes and quantitative by measuring the absorbance value
(AV) using an ELISA reader. Based on the research, it was found that the beta
esterase activity test of most mosquitoes showed moderate resistance to
organophosphates.
Keywords: dengue hemorrhagic fever, Aedes aegypti, organophosphate resistance,
beta esterase activity
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