CD Skripsi
Produksi Kalsium Karbonat Oleh Bakteri Ureolitik Dengan Variasi Kultur Bakteri Dan Media Pertumbuhan
Ureolytic bacteria are microorganisms that can produce urease enzymes and
precipitate calcium carbonate. Calcium carbonate is used as a self-healing
concrete agent, concrete construction material and bioremediation. This study
aims to determine calcium carbonate production from ureolytic bacteria, with
isolate SP. 32, SP. 34, SP. 48, SP. 83, and SP. 84 based on growth phase and
calcium carbonate production. Calcium carbonate was analyzed using Fourier
Transform Infra-Red (FTIR) for functional groups. The results obtained by
isolates SP. 84 in NB-U/Ca medium and SP. 34 in well water medium with the
fastest log phase two days of incubation time. The highest calcite production was
produced by SP. 84 (418 mg/100 mL) in NB-U/Ca medium and SP. 32 (185
mg/100 mL) in well water medium. FTIR analyses results showed that isolates
SP. 32, SP. 48, SP. 83, and SP. 84 had four absorption peaks of pure calcite
characteristics, 712, 874, 1792, and 2512 cm-1, while the isolate SP. 34 had three
absorption peaks of pure calcite characteristics, 872, 1791, and 2510 cm-1. These
five ureolytic bacteria could be developed as biocement candidates.
Keywords: ureolytic bacteria, calcite, NB-U/Ca medium, well water medium,
FTIR.
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