CD Skripsi
Keragaan Kedelai (Glycine Max.) Generasi M1 Hasil Mutasi Varietas Dega 1 Dengan Beberapa Konsentrasi Ethyl Methane Sulphonate
ABSTRACT
To increase soybean productivity required some efforts as one is to
develop new high yielding variety or improving existing varieties. To obtain and
improved a variety, one need to obtain a population with high genetic variability
in order to ease the selection of new suitable cultivars. In this study, we used
induce mutations using chemical mutagen, ie, Ethyl Methane Sulphonate (EMS),
to increase the variability. This research had been conducted in the Plant Breeding
Laboratory and Experimental Garden of the Riau University. Seed of Dega-1
Variety were soaked by solution of EMS in buffer phosphate within several
concentration ie, 200 ppm, 300 ppm, 400 ppm, 500 ppm, 600 ppm and 700 ppm.
Seed then were washed with aquadest to cleand remaining EMS solution and than
planted together by initial Dega-1 as control in the field experiment using a
completely randomized design (RAL) with four replications. Parameters observed
were plant height, number of internodes the age of flowering, age of harvest,
number of pods per plant, number of filled pods per plant, number of seeds per
plant, seed weight per plant, number of seeds per pod and type of growth, flower
color, and pod color. Population variance were calculated by Microsoft Excel
software. The homogeneity of the variance was tested with HOV in accordance to
the procedures of the Levene’s test. The results showed that the induction of
mutations with 200 ppm of EMS increased genetic variance in M1 generation
compare to Dega 1 varieti for most quantitative character, including plant height,
number of pods per plant, number of filled pods per plant, number of seeds per
plant, and seed weight per plant. Induce the mutation by EMS solution, on the
other hand, didn’t change all qualitative character.
Keywords: Induced Mutation, Ethyl Methane Sulphonate, Population Variance,
Quantitative Traits, Qualitative Traits
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