ABSTRACT Lactic acid bacteria (LAB) are commonly used to preserve food and extend the shelf life of food. Media de Man Rogosa and Sharpe (MRS) is the medium commonly used to grow LAB specifically. This study aimed to investigate the effect of adding yeast extract and sucrose in MRS medium on the Lactobacillus plantarum TMW 1.1623 acidity value, total LAB, antimicrobial activity of the LAB…