CD Skripsi

Pertumbuhan Dan Aktivitas Antimikroba Pediococcus Pentosaceus Strain 2397 Terhadap Pectobacterium Carotovorum Subsp. Carotovorum Pada Berbagai Sumber Nutrisi

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ABSTRACT
The addition of nutrient sources could increase lactic acid bacteria's
growth and antimicrobial activity against pathogenic and spoilage bacteria by
producing antimicrobial compound. Pediococcus pentosaceus strain 2397
produced bacteriocin with a molecular weight of 14.4 kDa. The purpose of this
study was to evaluate the growth and antimicrobial activity produced by
Pediococcus pentosaceus strain 2397 against the spoilage bacteria Pectobacterium
carotovorum subsp. carotovorum on various nutritional sources. This research
was conducted experimentally using a completely randomized design with ten
treatments and four replications. The treatments in this study were S1 = without
the addition of carbon and nitrogen sources, various sources of carbon (S2 =
addition of 2% fructose, S3 = addition of 2% glucose, S4 = addition of 2%
lactose, S5 = addition of 2% maltose and S6 = addition of 2% sucrose) and
nitrogen nutrients (S7 = addition of 2% ammonium sulfate, S8 = addition of 2%
yeast extract, S9 = addition of 2% beef extract and S10 = addition of 2% peptone),
each with a concentration of 2%. The data obtained were analyzed statistically
using analysis of variance and continued with Duncan's new multiple range test
(DNMRT) at the 5% level. The results showed that the source of carbon and
nitrogen nutrients 2% significantly affected the growth of Pediococcus
pentosaceus strain 2397 and antimicrobial activities of supernatant cell-free and
crude bacteriocin against Pectobacterium carotovorum subsp. carotovorum. The
S9 treatment had the largest absorbance value of 2.71 nm. In the S8 treatment
(addition of 2% yeast extract) the largest cell-free supernatant clear zone was
13.60 mm. The largest crude bacteriocin clear zone was obtained in S9 treatment
using 2% beef extract with an inhibition zone diameter of 15.07 mm.
Keywords: Pediococcus pentosaceus strain 2397, growth, antimicrobial activity,
Pectobacterium carotovorum subsp. carotovorum, source of nutrition

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1706113934

Penerbit

Pekanbaru : Universitas Riau –Fakultas Pertanian – Teknologi Pertanian., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1706113934

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

TEKNOLOGI PERTANIAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

Daus

Versi lain/terkait

Lampiran Berkas

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