CD Skripsi

Karakteristik Pertumbuhan Lactiplantibacillus Plantarum Tmw 1.1623 Pada Media Halal Menggunakan Pepton Ikan Patin Sebagai Sumber Nitrogen

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ABSTRACT
Commercial peptone is mainly unacceptable for Muslims because it contains elements or components prohibited by religious considerations. This study aimed to produce halal peptone from catfish fillet using papain enzyme as an alternative source of nutrition that supports the growth of Lactiplantibacillus plantarum TMW 1.1623 compared to commercial media. Peptone produced by enzymatic hydrolysis is 5% (w.v-1) papain carried out for 6 hours. The characteristics of chemical composition, yield, degree of hydrolysis, media acidity, amino acid content and bacterial growth rate were observed. Experimental studies used a completely randomized design (CRD) with four treatments and four replications. The research treatments were commercial MRS broth (IKY1) and modified commercial MRS broth using halal ingredients (IKY2, IKY3, and IKY4). The data obtained were statistically analyzed using analysis of variance (ANOVA) and Duncan’s multiple range test (DMRT) at a 5% level. The results of this research showed that catfish liquid peptone had a moisture content of 90.74%, ash 4.22%, protein 7.19%, a yield of 90.32%, degree of hydrolysis of 80.40%, and contained high values of amino acids such as glutamic acid 5.25%, lysine 3.61%, aspartic acid 3.06%. The best results were obtained with the addition of fish peptone, soy protein isolate hydrolyzed, and yeast extract (IKY3) at a ratio of 4.50:13.50:4. The medium acidity after incubation value was 4.47. The total LAB increased to 9.055 log CFU.ml-1 was higher than that with the commercial MRS broth 8.940 log CFU.ml-1. The results revealed that catfish fillet is promising alternatives material for bacterial peptone.
Keywords: Enzymatic hydrolysis, fish peptone, Lactiplantibacillus plantarum
TMW 1.1623, lactic acid bacteria, papain.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1606123680

Penerbit

Pekanbaru : Universitas Riau –Fakultas Pertanian– TEKNOLOGI PERTANIAN., 2022

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1606123680

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

TEKNOLOGI PERTANIAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

DAUS

Versi lain/terkait

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