CD Skripsi
Pemanfaatan Limbah Pengolahan Filet Ikan Patin Dan Empulur Nanas Sebagai Pepton Halal Untuk Pertumbuhan Mikroba
ABSTRACT
Peptone is an essential product in biotechnology and functional food. Commercial peptone needs consideration to use because not proven in halal aspects and could contain components prohibited by Islamic law considerations. This study aims to obtain the best interaction between the ratio of pangasius filet waste and pineapple core with hydrolysis time to produce halal peptone as an alternative nutrient to support the growth of Lactiplantibacillus plantarum TMW. 1.1623. Peptone was produced by mixing pangasius filet waste and pineapple core with a ratio of 1:1, 2:1, and 3:1 which was hydrolyzed for 24, 48, and 72 hours. Data were analyzed using analysis of variance (ANOVA) and Duncan's multiple range test (DMRT) at a 5% level. The results showed that mixing pangasius filet waste and pineapple core with a ratio of 3:1 which was hydrolyzed for 24 hours, produced the best fish peptone with peptone yield of 42.88%, protein 5.68%, DH 90.28%, pH 4.10 and LAB 9.27 Log CFU/mL. The results showed that peptone extracted from Pangasius waste filet production using bromelain enzymes from pineapple cores could be used as the nitrogen source and nutrients for LAB growth.
Keywords: Enzyme, protein hydrolysis, fish peptone, lactic acid bacteria, bromelain
Tidak tersedia versi lain