CD Skripsi
Lama Penyimpanan Pepton Halal Dari Limbah Filet Ikan Patin Pada Suhu Refrigerator Terhadap Pertumbuhan Bakteri Asam Laktat Dan Bakteri Patogen
ABSTRACT
Peptone is a protein hydrolysate that is easily damaged when stored at room temperature. This situation underlies the need for storage in the refrigerator to extend the storage length. The study aimed to obtain the optimal shelf life of halal peptones from catfish waste at a temperature refrigerator by looking at the growth characteristics of lactic acid and pathogenic bacteria inoculated on halal media. The research was conducted experimentally using a completely randomized design with four treatments and four replications. The treatment consists of T1, T2, T3, and T4 consecutive storage times for 0, 7, 14, and 21 days. The data obtained were analyzed statistically using analysis of variance (ANOVA) and further tests of Duncan's multiple range test (DMRT) at a level of 5%. The results showed that peptone storage at refrigerator temperatures was effective in the growth of lactic acid bacteria and pathogens up to the 21st day with total cells of Lactobacillus plantarum TMW 1.1623 (9.99±0.528 log CFU/ml), Streptococcus thermophilus (10.18±0.358 log CFU/ml), Escherichia coli 0157:H7 (10.39±0.150 log CFU/ml), and Listeria monocytogenes CFSAN004330 (10.51±0.180 log CFU/ml). Halal peptones made from catfish waste and pineapple pith have yield of 43.88±0.243%, pH of 5.11±0.006, protein content of 8.11±0.016%, and protein hydrolysis degree of 88.24±0.150%. Halal peptone from this study has 15 types of essential and non-essential amino acids dominated by glutamate as much as 6.04%, glycine 3.69%, aspartic 3.43%, and leucine 2.81%.
Keywords: Catfish waste, halal, lactic acid bacteria, peptone, pathogenic bacteria
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