CD Skripsi
Evaluasi Keamanan Probiotik Dan Aktivitas Antioksidan Lactobacillus Fermentum Inacc B1295 Yang Dienkapsulasi Cellulose Microfiber Dari Tandan Kosong Kelapa Sawit Selama Penyimpanan
ABSTRACT
Live microorganisms that have the potential as probiotics need to be evaluated for their safety and antioxidant activity to qualify as probiotics. The purpose of this study was to evaluate the safety and antioxidant activity of cellulose microfiber (CMF)-encapsulated probiotics from oil palm empty fruit bunches (OPEFB) during storage. This study was carried out experimentally consisted of safety evaluations analyzed descriptively and antioxidant activity statistically analyzed in a factorial complete randomized design. The experimental unit consisted of two factors, namely temperature (±25C and 4C) and storage time (0, 14, and 28 days) with three replications each to obtain 18 experimental units. Parameters observed in this study were safety evaluation (hemolytic activity, cytolysin production, gelatinase production) and antioxidant activity 1,1-diphenyl-2-picrylhydrazil (DPPH) radical scavenging and hydroxyl radical scavenging (HRS). The observed data of antioxidant activity were analyzed for variance and continued with the duncan multiple range test at 5% level. The results showed that lactic acid bacteria (LAB) encapsulated by CMF from OPEFB was able to maintain its safety properties with negative results in hemolytic activity, cytolysin production, gelatinase production test during storage at room (±25C) and cold temperature (4C). The storage temperature, storage time, and their interactions significantly affected (P
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