CD Skripsi
Struktur Jaringan Ikan Mas (Cyprinus Carpio) Yang Diberi Imunostimulan Tepung Daun Jambu Biji (Psidium Guajava)
Addition of guava (Psidium guajava) leaves powder in fish feed pellets has been used to improve the immunity of goldfish (Cyprinus caprio). However, there was anti-nutrients agents present in that leaves that negatively affects the food absorption as well as alter the fish health. To understand the effects of guava leaf powder provision and to determine the guava leaf powder dose that is safe for fish health, this study has been conducted. The health of the fish was studied through investigating the fish’s intestine, liver, and kidney structure. A one-factor completely randomized design (CRD) was applied. The guava leaf powder (GLP) was mixed with Hi-Pro-Vite 781-2 commercial fish feed pellet. There were 5 treatments applied, namely P0 (without GLP), P1 (1.0 g GLP/100 g feed), P2 (1.5 GLP/100 g feed), P3 (2.0 g GLP/100 g feed), and P4 (2.5 g GLP/100 g feed). The fishes were reared in floating fish net cage (4x4x7 m) placed in the Koto Panjang Dam. There were 30 fishes/ cage. The fishes were fed 3 times/day ad libitum and they were reared for 60 days. By the end of experiment, the intestine, liver, and kidney tissue were removed and histologically processed (alcohol series, paraffin embedded and Haematoxylin-Eosin stained). Results shown that provision of GLP enriched feed affects the intestine, liver, and kidney tissue of the fish in general. The least changes in tissue structure were found in the P2 (1.5 g/100 g feed) as there was no abnormality in the intestine. While in other treatments, there were abnormality such as congestion, villi damage, and decrement of the number of goblet cells. While in the liver and kidney of all fish treated in this research, there were abnormalities such as haemorrhage, necrosis, and tissue inflammation present in these organs.
Keyword: tissue structure, cyprinid fish, goblet cell, fish health status, anti-nutrient
Tidak tersedia versi lain