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Isolasi Senyawa Metabolit Sekunder Dan Uji Antijamur Dari Estrak N-Heksana Akar Kuning (Arcangelisia Flava L.)

traditional application by local communities in treating various diseases. Previous research has effectively isolated and characterized secondary metabolites from A. flava, demonstrating their potent antibacterial, antimalarial, antioxidant, and anticancer activities. This study aimed to isolate secondary metabolite compounds from A. flava and assess their antifungal activity in vitro and in silico methods. The extraction process of A. flava involved methanol and partitioning using n-hexane, dichloromethane, and ethyl acetate. To yield pure compounds, the n-hexane extract underwent further separation via chromatographic techniques, including vacuum liquid and flash chromatography. The purity of the three isolated compounds was verified through melting point determination, thin-layer chromatography, and high-performance liquid chromatography. Spectroscopic methods such as UV-visible, FTIR, and NMR spectroscopy were employed to analyze the structural characteristics of these compounds. Through structural elucidation, compounds 23-10-AK-FIII, 23-10-AK-FVII, and 23-10-AK-SFVIII were identified as β-sitosterol, daucosterol, and palmatine. The antifungal activity assays revealed the compounds' efficacy in inhibiting Candida sp. growth, with β-sitosterol and daucosterol demonstrating MIC (25 μg/mL) and MFC (100 μg/mL), and palmatin showing MIC (12.5 μg/mL) and MFC (50 μg/mL). Additionally, in silico analysis involving 14α-demethylase (PDB: 4LXJ) and 1,3-β-glucan synthase (PDB: 1EQC) receptors indicated a high affinity of these compounds towards both receptors. The findings suggest the isolated compounds from A. flava hold significant potential as candidates for antifungal drug development.

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Informasi Detail
Judul Seri
-
No. Panggil
2210246856
Penerbit
Pekanbaru : Universitas Riau Pascasarjana Tesis Pendidikan Kimia.,
Deskripsi Fisik
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Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
2210246856
Tipe Isi
-
Tipe Media
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Tipe Pembawa
-
Edisi
-
Subjek
PENDIDIKAN KIMIA
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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