CD Skripsi
Aktivitas Antibakteri Ekstrak Daun Bakau Minyak (Rhizophora Apiculata) Terhadap Bakteri Pseudomonas Aeruginosa Dan Bacillus Cereus
Rhizophora apiculata is a type of tropical mangrove that can survive in salty environments (halophytic). Mangrove leaf extracts generally contain phytochemical compounds that have the potential to be antibacterial. This study aims to determine the yield of R. apiculata leaf flour and extract, the content of phytochemical compounds of R. apiculata leaf extract, and the antibacterial activity of R. apiculata leaf extract including inhibition test; MIC (Minimum Inhibitory Concentration); and MBC (Minimum Bactericidal Concentration) with the methods of disc diffusion, liquid dilution, and solid dilution, respectively. The research data were analyzed descriptively. The parameters analyzed were yield, phytochemical compounds (qualitative) and antibacterial activity. The results showed that the yield value of R. apiculata leaf flour was 56.36%, while the yield of the thick extract was 15.93%. Phytochemical compounds contained in R. apiculata leaf extract are alkaloids, flavonoids, saponins, terpenoids, and tannins. The results of the antibacterial activity test showed that R. apiculata leaf extract has the ability to inhibit the growth of Pseudomonas aeruginosa and Bacillus cereus bacteria with inhibition power varying from weak to strong categories. The highest inhibition was found at a concentration of 100% which produced an inhibition zone of 12.88 ± 0.25 mm in P. aeruginosa bacteria and 15.42 ± 0.23 mm in B. cereus bacteria. The MIC values against P. aeruginosa and B. cereus bacteria were 6.25% and 1.56%, respectively, while the MBC value was not found until the highest concentration.
Keywords: Extract, methanol, Rhizophora apiculata, phytochemicals, antibacterial, inhibition zone, MIC, MBC
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