CD Skripsi

Isolasi, Identifikasi Morfologi, Dan Uji Aktivitas Enzim Hidrolitik Bakteri Termofilik Dari Sumber Air Panas Bukik Kili Koto Baru, Solok, Sumatera Barat

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Indonesia is a country with many hot springs, one of which is Bukik Kili Koto Baru, Solok, but has not been explored further. Bacteria originated from hot springs are very useful in the field of biotechnology because they are capable of producing thermostable enzymes such as amylase, cellulase, inulinase and lipases needed in the industrial world. The purpose of this study was to isolate, identify and test the enzyme activity of thermophilic bacteria originated from hot water sources Bukik Kili Koto Baru, Solok. This research used the pour plate isolation method with duplicate repetition with three temperature variations which were 45ºC, 50ºC, and 55ºC. The process of identifying the morphology was carried out macroscopically and microscopically to determine the genus of bacteria and disc diffusion method to determine the ability of bacteria to produce thermostable hydrolytic enzymes. Based on the isolation process, 16 isolates were obtained with each of the 6 isolates for temperatures of 45ºC, and 50ºC, and 4 isolates at a temperature of 55ºC, There were three genus obtained from identification in morphology, Bacillus, Thermus, and Pseudomonas, which are more dominated by Bacillus genus. Based on enzyme assay, 2 isolates were able to produce amylase enzymes with the largest clear zone ratio value in LBKURCC242 isolates from 45ºC is (2,70±0,30) and LBKURCC192 (2.95 ± 0.30) from 50ºC. The highest test for cellulase enzyme activity was isolates LBKURCC263 (4,81±0,44). The largest inulinase enzyme activity test was found in LBKURCC244 isolates with a ratio of 3,42±0,00. Based on the result isolates which had the optimum ability to produce amylase, cellulase, and inulinase enzymes in the three temperature variations were LBKURCC242 and LBKURCC243 isolates

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02. 119 (0026)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2019

Deskripsi Fisik

xiii, 48 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 02. 119 (0026)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

JAKA

Versi lain/terkait

Lampiran Berkas

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