CD Skripsi
Potensi Antibakteri Pada Kitosan Dari Karapas Udang Rama-Rama (Thalassina Anomala)
Mud shrimp (Thalassina anomala) is a fishery commodity which its production is abundant. Unfortunately, it has a low economic value because most of its body is consisting of carapace, which reaches 50-70% of its total body weight. The economic value of carapace waste could be increased by by extracting it to be a chitosan. Chitosan is produced through the stages of demineralization, deproteination and deacetylation. The aim of the research was to determine the ability of chitosan extracted from T. anomala carapace to inhibit the growth of pathogenic bacteria. Chitosan extracted from the shrimp carapace was showing its chemical characteristics, namely: the content of water 6.74%, ash 4.13%, nitrogen 7.5% and deacetylation degree 71%. Chitosan was dissolved in 1% acetic acid. Antibacterial activity testing was carried out by using the well diffusion method, conducted by the addition of chitosan at the concentration varied at 0%, 1%, 2%, 4% and 6%. The results showed that the chitosan had ablity to be the antibacteria against Salmonella thypi, Escherichia coli, Staphylococcus aureus and Bacillus cereus. It was showed by the clear zones formed around the well, and therefore, indicated the inhibition to the growth of these bacteria. The average of inhibition zone diameter for each bacterium was 6.9 ± 0.29 mm, 6.6 ± 0.21 mm, 9.4 ± 0.29 mm, and 7.6 ± 0.31 mm, respectively. The clear zone formed was indicating that the chitosan extracted from the shrimp T. anomala carapace could inhibit the growth of pathogenic bacteria at a concentration of 2%.
Keywords: Thalassina anomala, carapace, chitosan, pathogenic bacteria, antibacterial
1)Student of the Faculty of Fisheries and Marine Universitas Riau
2)Lecturer of the Faculty of Fisheries and Marine Universitas Riau
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