CD Tesis
Uji Resistensi Nyamuk Aedes Aegypti Terhadap Sipermetrin 0,05% Di Pelabuhan Sungai Duku Dan Bandara Sultan Syarif Kasim II Pekanbaru
Vector control must be carried out in the Port and Airport area. The use of chemical insecticides is one way to reduce the vector of Dengue Hemorrhagic Fever (DHF) caused by the Aedes aegypti mosquito. Given the use of different insecticides in an area will produce different resistance, it is necessary to test the resistance. This is in accordance with the Regulation of the Minister of Health of the Republic of Indonesia No. 50 of 2017 concerning Environmental Health Quality Standards and Health Requirements for Vectors and Disease-Carrying Animals and their Control, which states that vector control using insecticides must be carried out by insecticide susceptibility testing. Insecticide susceptibility testing in the Port and Airport area must be carried out at least 1 (once) a year. The use of Cypermethrin as an insecticide has been used since 2016 but has not been tested for its susceptibility status.
This study aims to determine the resistance status of Aedes aegypti mosquitoes to the insecticide Cypermethrin and to determine the difference in susceptibility of Aedes aegypti mosquitoes at Duku River Port and Sultan Syarif Kasim II Airport, Pekanbaru. This research method is descriptive with cross sectional design. This research was conducted in January - March 2020.
Eggs and larvae were collected from ovitrap and mosquito breeding sites around Duku River Port and Sultan Syarif Kasim II Airport in Pekanbaru. The eggs and larvae were then taken to the Laboratory for Environmental Risk Control, Pekanbaru Class II Port Health Office to carry out a 0.05% susceptibility test to Sipermetrin. The resistance test used WHO standard impregnated paper, an independent T test was performed to explain the differences in the vulnerability status of the two regions.
The results showed that Aedes aegypti at Duku River Port and Sultan Syarif Kasim II Airport in Pekanbaru were tolerant of Cypermethrin 0.05% with resistance yields of the two regions, respectively 83% and 81.25%. The emergence of tolerance due to genetic processes according to Darwin's law of selection that occurs in nature. Selection occurs because there is a small proportion of insects that have individual genetic mutations. This protective mechanism depends on genetic factors, either single, recessive, partly dominant or dominant in the hereditary process. If the individual insects are heterozygote, they rarely appear in the initial resistance process in an insect population including mosquitoes. However, the heterozygote that survives on the susceptibility test (susceptibility test) when it mates with another heterozygote will produce a homozygote proportion with a high level of resistance. If the homozygote resistance gene is dominant, the resistance will spread rapidly throughout the population.
Tolerance of Cypermethrin 0.05% against Aedes aegypti at Duku River Port and Sultan Syarif Kasim II Airport in Pekanbaru is also suspected to be due to biological factors. Cypermethrin can penetrate the chitin layer and through the
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lymph, and the lipids of the nerve channels will reach the insect's central nervous system.
Tolerance can also be caused by operational factors. The continuous use of insecticides at Duku River Port and Sultan Syarif Kasim II Airport in Pekanbaru, namely by spraying / fogging 4 times a year even though there is no dengue outbreak can lead to tolerance. According to the Head of the Environmental Risk Control section of the Class II Port Health Office Pekanbaru, routine fogging is carried out in an effort to achieve the mandate of the Indonesian Minister of Health No. 50 of 2017 concerning Environmental Health Quality Standards and Health Requirements for Vectors and Animals Carrying Disease and their control stating that the port and airport areas must be free from disease vectors.
The test of different vulnerabilities at Duku River Port and Sultan Syarif Kasim II Airport in Pekanbaru was carried out by comparing the data on the status of mosquito resistance in the two regions. The different test is calculated by statistical tests, namely the T Independent test. Prior to the independent T test, the data normality test was carried out and the data homogeneity test was carried out. From the calculation of the T value, the t count value is smaller than the t table (0.252
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