CD Skripsi

Ekstraksi Kolagen Dari Kulit Ikan Patin (Pangasius Sp.) Dengan Konsentrasi Enzim Papain Berbeda

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Abstract
This study aims to determine the effect of different papain enzyme
concentrations on collagen extraction from the skin of catfish (Pangasius sp.). The
method used was an experimental method with completely randomized concoctions
(CRD). The treatments given consisted of E0 (Control), E1 (5% papain enzyme), E2
(10% papain enzyme), E3 (15% papain enzyme). This research consisted of 3 stages,
namely 1) preparation of raw catfish, 2) deproteination of catfish skin, 3) hydrolysis of
raw catfish with acetic acid. The parameters tested were collagen yield, proximate
analysis (moisture content, ash content, and protein content), pH, and functional group
(FTIR). The results showed that the quality of the collagen obtained increased along
with the increase in the concentration of the enzymes used. This is presumably because
the addition of the enzyme concentration in the collagen extraction process will increase
the yield produced because the enzyme breaks the cross-linking of the collagen
telopeptide and dissolves it. Based on this, the use of the optimum concentration was
obtained at a concentration of 15% (E2) with a protein content of 71,60%, 14.17%
water, and 4.21% ash. Meanwhile, the results of functional group analysis using FTIR
produced peaks of absorption that include amida A, amida B, amida I, amida II and
amida III which showed that the resulting collagen extract has not been degraded into
gelatin.
Keywords: Collagen, Catfish, Extraction, Papain Enzymes.
1)Student of The Faculty of Fisheries and Marine Science, Universitas Riau.
2)Lecturer of The Faculty of Fisheries and Marine Science, Universitas Riau

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

04 04. 121 (0014)

Penerbit

Pekanbaru : Universitas Riau – Fakultas Perikanan Dan Ilmu Kelautan - Teknologi Hasil Perikanan., 2021

Deskripsi Fisik

ix, 77 hlm.; ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

04 04. 121 (0014)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

TEKNOLOGI HASIL PERIKANAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

Daus

Versi lain/terkait

Lampiran Berkas

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