ABSTRACT Ribu-ribu (Anisophyllea disticha (Jack) Bail) belongs to Anisophyllea family that usually grows tropical forests and used as traditional medicine by the Talang Mamak tribe to treat headache. This study aims were to isolate the secondary metabolites from the leaves of ethyl acetate extract as well as their toxicity level. Phytochemical analysis showed that the leaves contained saponins…