Kemampuan Bakteri Pelarut Fosfat Asal Bukit Batu Riau Dalam Menghasilkan Asam Sianida Dan Siderofor Serta Uji Hipersensitif Pada Tembakau (Nicotiana Tabacum)

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Kemampuan Bakteri Pelarut Fosfat Asal Bukit Batu Riau Dalam Menghasilkan Asam Sianida Dan Siderofor Serta Uji Hipersensitif Pada Tembakau (Nicotiana Tabacum)

PRISTIANA APRILLIA / 0903114227 - Nama Orang;

Phosphate solubilizing bacteria (PSB) are beneficial bacteria which arenot only capable of solubilize organic phosphate into inorganic phosphate, but also produce siderophore and cyanide acid (HCN). Siderophore plays arole as iron chelators, stimulating plant growth and controlling several plant diseases, while HCN is able to control weeds growth. The objectives of this research were to screen the potency of indigenous PSB isolated from peat soil in siderophore and HCN production and to determine its pathogenicity by using hypersensitive assay. The collections of PSB were subcultured in King’s B broth. The ability of 152 isolates on HCN production were tested qualitatively by using filter paper soaked with picric acid and Na2CO3. As many as 26 isolates (17.1%) were able to release various level of HCN production based on appearing of brown, dark brown, or reddish brown colour on the filter paper. The ability of isolates to produce siderophore was tested by growing isolates on CAS medium and incubated for 48 hours with a positive result is indicated by the formation of an orange zone around colony. As many as 15out of 92 isolates tested were able to produce siderophore. Based on hypersensitivity test, as many as 94 isolates showed a hypersensitive reaction in tobacco plants with the formation of a brownish color around tobacco leaves inoculated with those isolates.

Keyword : Cyanide acid, Hypersensitive reaction, Phosphate Solubilizing Bacteria (PSB), Siderophore, Tobacco plant

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Perpustakaan Universitas Riau 03 04.013 (0098)

03 04.013 (0098)

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Judul Seri: -
No. Panggil: 03 04.013 (0098)
Penerbit: Pekanbaru : Universitas Riau - FMIPA - Biologi., 2013
Deskripsi Fisik: xii, 39 hlm.;ill.; 29 cm
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Klasifikasi: 03 04.013 (0098)
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Pernyataan Tanggungjawab: Dewa

Kemampuan Bakteri Pelarut Fosfat Asal Bukit Batu Riau Dalam Menghasilkan Asam Sianida Dan Siderofor Serta Uji Hipersensitif Pada Tembakau (Nicotiana Tabacum)

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