CD Skripsi
Viabilitas Bakteri Asam Laktat Yang Diisolasi Dari Fermentasi Sap Sawit Pada Asam Hidrochloric Dan Garam Empedu Secara In Vitro
Lactic acid bacteria from fermented palm sap can potentially be developed as probiotics. Probiotic LAB candidates should be capable of withstanding the gastrointestinal tract and colonizing the intestinal surface. Probiotic LAB candidates must be able to survive in the presence of acids and bile salts. This study aimed to determine the viability of LAB isolated from fermented palm sap against hydrochloric acid and bile salt (oxgall) by in vitro. The treatments in this study were five LAB isolates A1, A6, B12, C1, and C4 isolated from fermented palm sap. This study used MRS broth adjusted with HCL and MRS broth containing oxgall. The isolates viability data was tabulated and analyzed descriptively. The results showed that isolates A1, A6, B12, C1, and C4 survived at pH 2, 2.5, and 3 for 90 and 180 minutes of incubation with viability percentages ranging from 63.46–100.48% at pH 2, 65.12–151.97% at pH 2.5, and 82.13–118.39% at pH 3. Isolates A1, A6, B12, C1, and C4 survived and were able to grow well in MRS broth medium containing 0.3 and 0.5% oxgall for 180 and 300 minutes incubation with viability percentages ranging from 100.17–180.40% at concentration 0.3% oxgall and 82.09–161.56% at 0.5% oxgall. From this study, it was concluded that five LAB isolates displayed high tolerance to acidic conditions and bile salt and had potential as probiotics candidates, because they had viability >50% on low pH and bile salt.
Keywords: lactic acid bacteria, viability, hydrochloric acid, oxgall
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