CD Skripsi

Analisis Multilokus Menggunakan Barkode Dna Matk, Atpb-Rbcl Intergenic Spacer, Dan Trnl-Trnf Intergenic Spacer Pada Famili Pandanaceae

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The Pandanaceae family comprises trees, shrubs, and woody lianas. Most species in the Pandanaceae family, particularly those in the genus Pandanus, exhibit morphological variations, posing challenges in species differentiation. This study aims to perform multilocus analysis using DNA barcode sequences of matK, atpB- rbcL IGS, and trnL-trnF IGS in the Pandanaceae family. The matK, atpB-rbcL IGS, and trnL-trnF IGS sequences were collected from GenBank and aligned using MAFFT v.7. The aligned sequences were then concatenated into multilocus sequences, followed by data frequency analysis and the construction of a Neighbor Joining phylogenetic tree using MEGA11 software. The results showed that the matK sequence exhibited the highest genetic stability with 91% conserved sites, making it suitable for identifying broader species groups. In contrast, the trnL-trnF IGS sequence displayed the highest genetic variation at 32.4%, while atpB-rbcL IGS also demonstrated significant variation, making both effective for distinguishing morphologically similar species. Based on the phylogenetic tree structure, some species within the Pandanaceae family could not be well-clustered using unilocus and bilocus sequences. However, the use of multilocus sequences resulted in a phylogenetic tree with well-clustered species. Therefore, the multilocus combination of atpB-rbcL IGS + matK + trnL-trnF IGS significantly enhances species differentiation within the Pandanaceae family and reduces errors in taxonomic identification compared to unilocus and bilocus sequences.
Key findings: Pandanaceae family, multilocus analysis, phylogenetic tree, taxonomic identification

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2103110334

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2103110334

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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