CD Skripsi
Karakterisasi Fitokimia Daun Ampelai Kijang (Tetracera Scandens (L.) Merr.) Menggunakan Lc-Ms Dan Uji Toksisitasnya Dengan Metode Bslt
Tetracera scandens (L.) Merr. That commonly known as ampelai kijang, is a plant traditionally used to treat respiratory disorders, hepatitis, diabetes, and HIV infections. In addition to its cultural significance, this plant contains various secondary metabolites with pharmacological potential. However, studies on its active compounds and applications is limited. This study aimed to characterize the phytochemical content of T. scandens leaf extract using Liquid Chromatography- Mass Spectrometry (LC-MS) and to evaluate its toxicity by using the Brine Shrimp Lethality Test (BSLT). Extraction was performed using maceration with 70% ethanol. LC-MS analysis identified 27 secondary metabolites with retention times range from 1.44 to 17.93 minutes. The dominant compounds included flavonoids, terpenoids, and alkaloids, all of which are known for their biological activities such as antioxidant, antibacterial, and anti-inflammatory properties. Shoyuflavone A, a flavonoid, was detected in relatively high concentration. The BSLT assay using Artemia salina larvae revealed an LC50 value of 37.3936 ppm, which falls into the highly toxic category. These findings indicate a correlation between the identified phytochemical components and the extract’s toxic effects, suggesting its potential to be developed as an anticancer agent.
Key findings: Anticancer, LC-MS, Phytochemicals, Tetracera scandens (L.) Merr., Toxicity
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