CD Skripsi

Analisis Sekuens Nbs-Lrr (Nucleotide Binding Site-Leucine Rich Repeat) Pada Pisang Awak Dan Pisang Lilin

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Fusarium oxyforum f. sp cubense (Foc TR4) is agent of Fusarium wilt disease that significantly threaten banana existence. Two banana cultivars, awak and lilin, have been previously reported potential resistant against Fusarium wilt disease. NBS- LRR (Nucleotide Binding Site-Leucine Rich Repeat) gene is the largest group of R genes that play an important role in disease resistance. Purpose of this study was to analyze the NBS-LRR sequences of awak and lilin banana. In this study, total genomic DNA was extracted from young leaf tissues. Electrophoresis result from the two samples showed an amplicons size of 500 bp using NBS F and NBS R primer pair. Subseguently, the PCR product of two banana samples were sequenced. In addition, nucleotide bases were converted into deduced amino acid sequences. The result of this study showed that deduced amino acid sequences of pisang awak and pisang lilin contained NB-ARC domains and P-loop motif. The sequences obtained in this study have the potential as a resistant genetic markers in banana.

Key findings : Banana, Fusarium, Foc TR4, NBS-LRR, R genes

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2103111180

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2103111180

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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