CD Skripsi

Dereplikasi Senyawa Aktif Antimikroba Ko-Kultur Fermentasi Cair Penicillium Sp. Lbkurcc34 Dan Staphylococcus Aureus Menggunakan Analisis Metabolomik Berbasis Lc-Ms/Ms

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Penicillium sp. LBKURCC34 is a fungus that can produce secondary metabolite compounds which have antimicrobial activity against several pathogens, such as Staphylococcus aureus, Escherichia coli, and Candida albicans. Research on the identification of active compounds from liquid fermentation of ethyl acetate extract of the fungus Penicillium sp. LBKURCC34 has not been isolated and identified further down to the structure of the active compound. Further exploration of co-culture fermentation of Penicillium sp. LBKURCC34 with the pathogenic bacteria Staphylococcus aureus showed increased activity than single fermentation. The dereplication approach is a metabolomics analysis process where the extract is analyzed by LC-MS/MS and chromatogram data is identified to view a comprehensive metabolite profile. This study aims to analyze the antimicrobial active compound of the ethyl acetate extract (EtOAc) co-culture of Penicillium sp. LBKURCC34 and Staphylococcus aureus using a dereplication approach with LC-MS/MS based metabolomics analysis of the active fractions (F3, F4, F5, F6A, and F6D) and analyze the correlation of compounds from the active fractions with antimicrobial activity. The active fractions were analyzed using a UHPLC Q Orbitrap HRMS MS machine. The MS analysis results from UHPLC Q Orbitrap HRMS were further analyzed through metabolomics using Xcalibur, MS-DIAL, and the massIVE GNPS server. Multivariate analysis to see the correlation between the compounds produced and the activity data were examined using XLSTAT. The results of the Xcalibur analysis showed that 19 compounds from five active fractions were identified based on the dominant peak. Analysis using MS-DIAL and GNPS each produced compound content data for 8 metabolites and 4 compounds. There is one same compound identified from the GNPS, X-Calibur and MS-DIAL analysis, namely Cyclo (Val-Pro) with m/z
197.13. The compounds of Visnagin, 1-{4-[(3S)-3-(5-Phenyl-1,3,4-oxadiazol-2- yl)1- pyrrolidinyl]1-piperidinyl} ethenone and citrinin compounds have a strong correlation with anti-Candida albicans activity.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2003111263

Penerbit

Pekanbaru : Universitas Riau FMIPA Kimia., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2003111263

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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