CD Skripsi

Deteksi Sidik Jari Dna Poliketida Sintase Dan Kloning Gen Amilase Pada Mikroba Simbion Spons Laut Rhabdastrella Globostellata

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This research aims to determine the genetic potential of microbial symbionts sponge Rhabdastrella globostellata through the detection of DNA fingerprints for the biosynthesis of polyketides and hydrolase gene cloning of microbial symbionts in marine sponge Rhabdastrella globostellata. Detection of the encoding PKS gene by using Polymerase Chain Reaction (PCR) and using specific primers Trans-F and reverse KSHGTGR that produces the amplicon of 500 bp, whereas amylase gene cloning by using agarose Low Melting Point (LMP) 1% (w/v), which produces the amplicon size is 5 kb and 20 kb. DNA fragments from clones 32 (500 bp) encoding the Trans-F that have been successfully cloned into Escherichia coli DH5a cells and expressed sequence similarity with KS domain of about 61%. DNA fragments of 5 kb and 20 kb coding amylase gene has been successfully cloned into Escherichia coli DH5a cells with blue-white screening and the results of DNA 5 kb and 20 kb clones showed growth of white colonies, which showed antibacterial activity with the establishment of clear zone. Key words: Antibacteria, Metagenomics, Polyketide Syinthase (PKS), Polymerase Chain Reaction (PCR)

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

04 03. 116 (0019)

Penerbit

Pekanbaru : Universitas Riau - Fakultas Perikanan Dan Ilmu Kelautan - Ilmu Kelautan., 2016

Deskripsi Fisik

xi, 53 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

04 03. 116 (0019)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

ILMU KELAUTAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

DAUS

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