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Isolasi Dan Seleksi Bakteri Endofit Dari Akar Mangrove Di Kabupaten Bengkalis Sebagai Sumber Senyawa Antibakteri

Mangrove plants have adapted to a unique habitat with muddy saline waters, anaerobic soil, brackish tidal activities and high microbial and faunal competition. Leaves, stems, bark, roots and fruits of mangrove plants are a valuable resource that can be utilized for folk medicine. Mangrove plants harbor an extremely diverse endophytic bacterial flora, and are known as sources of new bioactive compounds. The purpose of this experiment were to isolate bacterial endophytes residing mangrove roots and conducted antagonistic test for these isolates in inhibit the growth of pathogenic bacteria. A total of 95 isolates ofendophytic bacteria have been isolated from mangrove plants identified as Bruguiera sp. and Ceriops tagal (Perr.) C.B. Rob. Antagonistic tests against 3 pathogenic bacteria i.e., Vibrio alginolyticus, Escherichia coli and Staphylococcus aureus showed that 3 isolates (isolate No. 34, 88, and 89) were able to inhibit the growth of Vibrio alginolyticus with diameter of clear zone of (11,3±0,5) mm; (9,1±0,5) mm and (8,9±0,1) mm, respectively. Two isolates (isolate No. 34 and 39) inhibited Staphylococcus aureus with diameter of clear zone of (9,7±0,4) mm and (10±0,5) mm, respectively. One isolate (isolate No. 34) showed a potential to produce broad spectrum metabolite because it able to inhibit the growth of Gram negative bacteria (Vibrio alginolyticus) and Gram positive bacteria (Staphylococcus aureus), while there were no endophytic bacterial had the ability to inhibit the growth of Escherichia coli. Based on the results of characterization with Gram staining, those endophytic isolates were Gram positive bacteria (1 coccus isolate and 3 other bacillus isolates).

Ketersediaan
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Perpustakaan Universitas Riau 03 02.119 (0020)
03 02.119 (0020)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
03 02.119 (0020)
Penerbit
Pekanbaru : Universitas Riau – FMIPA – Kimia.,
Deskripsi Fisik
xiii, 44 hlm.: ill.: 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
03 02.119 (0020)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
KIMIA
Info Detail Spesifik
-
Pernyataan Tanggungjawab
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Tidak tersedia versi lain

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