CD Skripsi

Penapisan Jamur Endofit Dari Tanaman Sagu Yang Berpotensi Antagonis Terhadap Ganoderma Boninense Penyebab Penyakit Busuk Pangkal Batang Kelapa Sawit

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ABSTRACT

Endophytic fungi as biological control method has been widely studied recently to control patogenict fungi such as Ganoderma boninense the cause of basal stem rot disease of palm oil plant. One of endophytic fungi resources is sago plant wich has not been much explored. This study aims to screen the endophytic fungi of sago plants which have the best antagonistic potency as biological agents against Ganoderma boninense the cause of stem rot disease of palm oil plant, The study was conducted at the Laboratory of Plant Disease, Faculty of Agriculture, University of Riau, Pekanbaru. The research used the exploration, observation and experimental methods. The research consisted of 7 steps: isolation and purification of endophytic fungi of sago plant, hipovirulent test of the endophytic fungi, the antagonistic test against G. boninense, diameter and velocity growth of the higher antagonistic endophytic fungi, hyperparasitism test of higher antagonistic endophytic fungi against G. boninense, inhibition test of secondary metabolite compounds against G. boninense and characterization of the higher endophytic fungal isolates. In experimental methods, including: antagonistic test of 20 isolates, 6 higher antagonist test, the diameter and the speed and the inhibition secondary metabolic compounds of the 6 higher isolates. The units of the research were arranged in a Completely Randomized Design (CRD). Data were analyzed descriptively and analysis of variance and further analyzed with Duncan's new multiple range test (DNMRT) at 5% level. The results found 20 isolates, 14 isolates were hypovirulents and 6 isolates were virulent. Isolate E12 had the highest antagonisticity (79.32%). The largest diameter and the most speed growth of the endophytic fungi were isolates E14 (90.00 mm and 53.37 mm/day). The hiperparasitic interaction of the higer antagonistic endophytic fungi were attachment (isolate E13), twist (isolates E10, E14), trapping (isolate E1) and lysis (isolates E1, E12). The highest inhibition of the secondary metabolic compounds was isolates E12 (5.28%). The results showed that isolates E1, E3 and E12 belong to the genus Trichoderma, isolates E10 belongs to genus Chrysosporium, isolates E13 and E14 belongs to genus Thielaviopsis.
Keywords : Screening, Sago plants , Endophytic fungi , G. boninense.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

06 01. 119 (0123)

Penerbit

Pekanbaru : Universitas Riau – Fakultas Pertanian – Agroteknologi., 2019

Deskripsi Fisik

vi, 71 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

06 01. 119 (0123)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Agroteknologi

Info Detail Spesifik

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Pernyataan Tanggungjawab

DAUS

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