CD Skripsi
Isolasi Dan Identifikasi Bakteri Penghasil Biosurfaktan Pada Lumpur Kolam Anaerob Ipal Industri Minyak Sawit
Abstract
In an effort to optimize the use of biosurfactant bacteria in the aquatic
environment in Indonesia, especially in Riau, the search for local strains bacteria
that have high capacity to degrade oil pollution in waters is highly desirable. One
of the places where biosurfactant bacteria live and develop is in the palm oil
industry's Wastewater Treatment Plant (WWTP). This research was conducted in
August - September 2019. Sampling was carried out on anaerobic pond mud at the
WWTP of the palm oil mill located at PT. Eka Dura Indonesia, Ujung Batu,
Rokan Hulu, Riau Province. Bacteria sample multiplication, isolation,
purification, emulsification, and biochemical tests were carried out at the
Bacteriology Laboratory of the Environmental Health Unit of Riau Province.
There were 6 bacteria obtained, with different morphology, namely 3 on Blood
agar and 3 on Mac Conkey Agar. Based on the results of the emulsification test,
among the six bacterial isolates, three bacteria produced biosurfactants, namely
isolates with the code M 1-5, Ba 1-4, and Ba 1-6. After biochemical tests
identification, it was found that the three isolates were Enterobacter aerogenes,
Proteus vulgaris, and Proteus mirabilis with isolate code Ba1-6 . It has the highest
emulsification index, namely Proteus mirabilis (31.25%). After identifying
bacteria using PCR 16S Ribosomal RNA method, it was found that Proteus
mirabilis bacteria had a similarity level to Proteus mirabilis Strain 13A 16s with a
percentage of 99.93%.
Keywords: Biosurfactant Bacteria, Oil Contamination, Proteus mirabilis.
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