CD Tesis

Isolasi Metabolit Sekunder Dan Uji Aktivitas Penghambatan Α-Glukosidase Dari Tumbuhan Putri Malu (Mimosa Pudica Linn)

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Mimosa pudica is a wild plant with nomerous bioactivities that is used in
traditional medicine in several countries. One of the bioactivities studied is the
potential of M. pudica as an antidiabetic drug. The aim of this study was to isolate
secondary metabolites from M. pudica and to evaluate their bioactivity against α-
glucosidase enzyme in vitro and in silico. M. pudica was extracted with methanol
and partitioned to produce n-hexane, dichloromethane, ethyl acetate, and water
extracts. The ethyl acetate was subjected to vacumn liquid chromatography (VLC)
to obtain 17 fractions. Fractions 20-06-C-FV and 20-06-C-FVII were tested for
their purity and characterized by UV-Vis, FTIR and NMR spectroscopy. Then, the
extracts and compounds were tested for α-glucosidase enzyme inhibition to
evaluate their inhibitory activity. Elucidation structure of 20-06-C-FV and 20-06-
C-FVII compounds identified as quercetin (48) and avicularin (13). Evaluation of
α-glucosidase enzyme inhibition activity showed that ethyl acetate extract
depicted the highest inhibition of 69.90% at concentration 500 ppm. Furthermore,
compound 48 demonstrated high activity when compared to acarbose, with IC50
values of 47.06 μg/mL and 129.58 μg/mL, respectively, whereas compound 13
demonstrated less activity with an IC50 of 230.62 μg/mL. Quercetin (48) and
avicularin (13) compounds were also tested by in silico using α-glucosidase
homolog modeling crystal structure receptor from S. cerevisiae (PDB ID 3A4A).
The molecular docking results showed that avicularin (13) compound had the
highest binding energy of -8.44 kcal/mol. Quercetin (48) also showed a binding
energy of -7.43 kcal/mol which is higher than acarbose as a drug standard which
has a binding energy of -6.51 kcal/mol. The conclusion of this study shows that
the isolated compounds of M. pudica have the potential to be used as antidiabetic
drug candidates.
Keywords: Antidiabetic, molecular docking, M. pudica

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2010241741

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Kimia., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2010241741

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

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