CD Skripsi
Histopatologi Insang Dan Hati Ikan Jambal Siam (Pangasianodon Hypophthalmus) Yang Diberi Pakan Mengandung Larutan Biji Alpukat (Persea Americana Mill)
Abstract
Avocado seeds are one of the natural ingredients that contain several antibacterial compounds such as polyphenols, flavonoids, triterpenoids, quinones, monoterpenoids, sesquiterpenoids, and alkaloids. This research was conducted in May-July 2022 at the Faculty of Fisheries and Marine, Riau University. The purpose of this research was to analyze changes in the histopathological structure of the gills and liver Pangasianodon hypophthalmus fed with avocado seed enriched pellets and challenged with Aeromonas hydrophila. This study used a completely randomised design (CRD) method with five treatments and three replications so that 15 experimental units were obtained. The treatments were (Kn) negative control (feeding without avocado seed solution and without infection with A. hydrophila bacteria), (Kp) positive control (feeding without avocado seeds enriched with A. hydrophila) and feed with the addition of avocado seeds enriched and infected with A. hydrophila at a dose of 1,000 ppm (P1), 1,150 ppm (P2) and 1300 ppm (P3). Pangasianodon hypophthalmus used in the study had a size of 8-12 cm and were reared for 46 days. Gill and liver were taken 2 times namely 30th and 46 th day infection. Organ samples were fixed using 10% formalin. Histological preparations with HE staining. The results shown that the P3 (1300 ppm) was the best dose with P < 0.005 as seen from the gill lamella distance around 0.35 mm and the level of damage to organs (HAI) in the gill based on data obtain structure was shown normal condition. Water quality parameters during the study were temperature 27-29 0C, pH 5-7 DO 3.3-4.7 mg/L, and ammonia 0.018-0.028 mg/L. It can be concluded that the addition of avocado seeds in is able to improve the tissue organ of Pangasianodon hypophthalmus on reducing damage to the gills and liver caused by A. hydrophila.
Keyword: Gills, Liver, Aeromonas hydrophila, Pangasianodon hypophthalmus, Avocado seeds
1) Student of Faculty of Fisheries and Marine, Riau University
2) Lecturer of Faculty of Fisheries and Marine, Riau University
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