CD Skripsi

Analisis Metabolomik Dan Kemometrik Berbasis Lc-Hrms Dari Ko-Kultur Fermentasi Cair Penicillium Sp. Dan Trichoderma Spp

---

Infections caused by pathogenic microorganisms are increasing, resulting in increasing cases of antibiotic resistance. So this has become an important focus for researchers to find new candidate compounds that have antibacterial and antioxidant activity. One of the fungi isolated from local Riau is Penicillium sp. LBKURCC34 and Trichoderma spp. LBKURCC1 and LBKURCC2. These fungi produce secondary metabolites that have potential antibacterial and antioxidant activities, but it is not yet known what compounds play a role in these activities. This study aims to find candidate compounds from the active extracts of ethyl acetate and butanol from the co-culture of Penicillium sp. LBKURCC34 and Trichoderma spp. LBKURCC1 and LBKURCC2 that can be used as antibacterial and antioxidant with target and nontarget approaches, which are carried out comprehensively with XCalibur, MS-DIAL, MS-FINDER, GNPS and XLSTAT software. The active extract of the co-culture was analyzed using LC-HRMS Q Orbitrap, the raw data generated was analyzed for compounds based on the database available on the tool using XCalibur, followed by data processing using MS-DIAL software. Then data filtering is carried out to determine potential metabolites. The same raw data was also analyzed using GNPS to see the molecular network. Furthermore, multivariate analysis was carried out using XLSTAT to see the proximity correlation between active extracts. Based on the analysis conducted on MS-DIAL and MSFINDER, 37.849 metabolites were annotated before filtering, and 292 metabolites after filtering. Furthermore, data matching of compounds identified in XCalibur with MSDIAL and MSFINDER was carried out as many as 5 compounds, namely (NP-011220 (Cyclo(-leu-pro)), N-acetyltyramine, Hexa- methoxy-methyl-melamine, Oleamide, Glycocholic acid). Furthermore, a correlation analysis was carried out on the identified compounds with their activity data using XLSTAT, obtained as many as 5 compounds that were strongly correlated with antibacterial and antioxidant activities, namely (N-(2 phenylethyl) acetamid, NP-011220 (cyclo(-leu-pro) N-acetyltyramine, hexa-methoxy-methyl- melamine, and oleamide).

---

Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

2003111262

Penerbit

Pekanbau : Universitas Riau FMIPA Kimia., 2024

Deskripsi Fisik

-

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

2003111262

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

KIMIA

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar