CD Skripsi

Isolasi Dan Uji Aktivitas Antijamur Senyawa Metabolit Sekunder Dari Fraksi 3 Ekstrak Kloroform Tumbuhan Paku Sarang Burung (Aplenium Nidus L.)

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Asplenium nidus, commonly known as bird's nest fern, belongs to the Aspleniaceae family. This fern typically grows in both lowland and highland areas, characterized by a single leaf and a rosette-shaped group of leaves, and it cannot tolerate direct sunlight. This study aims to isolate and characterize compounds from the chloroform extract of A. nidus and evaluate its antifungal activity using the microdilution method against Candida species. Isolation was conducted using the maceration method with methanol solvent, followed by partitioning with n-hexane, chloroform, and ethyl acetate. The crude chloroform extract was separated using vacuum liquid chromatography (VLC), resulting in 17 fractions, which were then combined into 6 main fractions. Fraction 4 was further separated using flash column chromatography, yielding 357 vial, which were then combined into 12 main subfractions. Subfraction 10, containing precipitates, was recrystallized using ethyl acetate, and its purity was analyzed using TLC, melting point, and HPLC analysis. Crystals from subfraction 10 were coded as 23-12-B- FIII-SF10-185/335, with a melting point of 312-314°C. This compound was elucidated using UV-Vis, FTIR, and NMR spectroscopy, and identified as luteolin. The MIC values of compound 23-12-B-FIII-SF10-185/335 against the fungi C. albicans, C. glabrata, and C. krusei were determined using the microdilution method, with respective MIC values of 100 μg/mL, 50 μg/mL, and 25 μg/mL, while the MFC values for C. albicans, C. glabrata, and C. krusei were
> 1000 μg/mL.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2003110686

Penerbit

Pekanbaru : Universitas Riau FMIPA Kimia., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2003110686

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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