CD Skripsi

Isolasi Dan Uji Toksisitas Senyawa Metabolit Sekunder Dari Ekstrak Etil Asetat Tumbuhan Paku (Davallia Denticulata)

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Davallia denticulata is a species of the Davalliaceae family and one of the epiphytic ferns that grows on oil palm. Several species of this genus are reported to have antioxidants, antimicrobials, and anticancer properties. This study aims were to isolate secondary metabolites from ethyl acetate extract of D. denticulata and its toxicity analysist as well ass its fractions. Phytochemical analysis showed that D. denticulata contained of flavonoids, terpenoids, steroids and saponins. Ethyl acetate extract was obtained from maceration and continued with VLC to produce 12 fractions. Fractions 5, 6, and 7 were combined and carried out with flash chromatography separation and yielded 50 subfractions. Crystals were obtained from subfraction 15 to 19 and recrystallized. White crystals were coded DD-EE-01 and analyzed their purity using TLC and the melting point test showed 118-120 ᵒC and the FT-IR data shows similarities with β-sitosterol. Furthermore, subfraction 20 to 50 were then combined and separated by flash chromatography and resulting of bright pink solid (DD-EE-02). This compound was analyzed for its purity by using TLC and the melting point showed 92-94ᵒC. The FT-IR spectrum shows the presence of C-H aliphatic groups, C-O, and C=O and GC-MS data with m/z 261. Based on the data obtained, the compound is proposed as fatty acid methyl esters. In addition, the toxicity analysis of extracts and fractions
(5, 6, 7) using BSLT showed various LC50 value of 119,80 ppm , 281,92 ppm, 330,07 ppm and 385,35 ppm, respectively.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 02. 119 (0078)

Penerbit

Pekanbaru : Universitas Riau – FMIPA – Kimia., 2019

Deskripsi Fisik

xi, 31 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 02. 119 (0078)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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