CD Skripsi

Analisis Filogenetik Nibung (Oncosperma Tigillarium (Jack) Ridl.) Asal Kabupaten Bengkalis Berdasarkan Sekuen Gen Rbcl

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ABSTRACT
Nibung (Oncosperma tigillarium (Jack) Ridl.) is a type of palm that belongs to the family Arecaceae, subfamily Arecoideae. Nibung is widely used by coastal communities in Bengkalis Regency. The high utilization of nibung without conservation efforts and the absence of a conservation status that stipulates nibung as a protected plant makes the number in nature less, so that if left unchecked it can lead to extinction. Taxonomic studies using morphological markers on nibung are difficult to do, because generative characters such as flowers and fruits as important characters are difficult to find in the out of season period. Thus, an alternative analysis using molecular methods is required. The study aimed to analyze kinship relationships and to reconstruct phylogenetic trees based on the character of the rbcL gene. DNA Extraction Procedure used CTAB method with modification. DNA fragments were sequenced at the First Base Laboratories Malaysia through PT. Genetika Science Indonesia followed by alignment using Bioedit application and phylogenetic analysis using Maximum Parsimony (MP) and neighbour joining (NJ) methods in PAUP* application version 4.0a169. The results of the MP analysis grouped O. tigillarium (Bengkalis Island/ B1) and O. tigillarium (Rupat Island/ R1) to form a sister group clade with a bootstrap value of 100% and form a monophyletic clade with O. tigillarium (Bengkalis Island/B2), meaning that the three collections O. tigillarium is closely related based on the rbcL gene marker. The results of the NJ analysis showed that O. tigillarium (Bengkalis Island/ B2) was more primitive than the other two nibung collections because it had the longest genetic distance.
Key Words: Phylogenetic, CTAB method, Oncosperma tigillarium, rbcL

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

03 04. 121 (0027)

Penerbit

Pekanbaru : Universitas Riau - Fakultas Matematika Dan Ilmu Pengetahuan Alam - Biologi., 2021

Deskripsi Fisik

ix,112 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

03 04. 121 (0027)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

ELFITRA

Versi lain/terkait

Lampiran Berkas

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