CD Skripsi

Analisis Empat Barkode Dna Pada Famili Myrtaceae

---

The Myrtaceae family is one of the most diverse plant families and possesses significant economic value, primarily due to the presence of essential oils in its leaves, twigs, flowers, and fruits. However, overlapping morphological similarities among species within this family make identification based solely on morphological characteristics difficult. This study aims to analyze four DNA barcodes rbcL, matK, trnL-trnF Intergenic Spacer, and Internal Transcribed Spacer (ITS) both individually (unilocus) and in combination (multilocus), to identify species within the Myrtaceae family and determine the most effective barcode combination. This research was conducted using DNA sequences retrieved from GenBank and analyzed through bioinformatics software, including MAFFT for sequence alignment, BioEdit for sequence trimming, and MEGA for phylogenetic analysis. The results showed that, in unilocus analysis, ITS displayed the highest nucleotide variation, the greatest number of segregating sites, and the highest transition/transversion ratio, making it the most informative among the tested barcodes. In contrast, rbcL was found to be the most conserved. The combination of matK + rbcL + ITS + trnL-trnF IGS yielded the highest genetic diversity, providing the most accurate taxonomic resolution in the multilocus analysis. Phylogenetic tree construction revealed consistent clade separation according to genus, such as Syzygium, Eugenia, and Backhousia, although some overlap was observed between Myrcia and Marlierea. These findings emphasize that multilocus barcode analysis, particularly those involving ITS, offers more precise species identification and phylogenetic resolution compared to unilocus approaches.
Key findings: DNA Barcode, Internal Transcribed Spacer (ITS), matK, Myrtaceae, rbcL, trnL-trnF Intergenic Spacer.

---

Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

2103111475

Penerbit

Pekanbaru : Universitas Riau FMIPA Biologi., 2025

Deskripsi Fisik

-

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

2103111475

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

BIOLOGI

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar