ABSTRACT Periphyton is a microscopic organism that lives attached to a submerged substrate. This study aims to determine the trophic status based on periphyton on a glass substrate at Lake Perupuk conducted in June-July 2019. There are three sampling stations, namely S1 (at the inlet lake), S2 (in the middle of the lake) and S3 (at the end of the lake). Sampling is done once/week for 3 wee…