CD Skripsi

Isolasi Dan Uji Toksisitas Senyawa Metabolit Sekunder Ekstrak Metanol Dari Kulit Batang Kelor Moringa Oleifera

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SUMMARY
Moringa oleifera is a plant that belongs to the Moringaceae family. This plant grows quickly and its resistant to drought, therefore it can grow in tropical, subtropical and arid regions. This study aims to isolate, characterize the pure compounds obtained from the methanol extract of M. oleifera stem bark and analyze its toxicity using the BSLT method. Isolation was carried out using the maceration method with methanol as a solvent and the crude methanol extract obtained was separated using vacuum liquid chromatography to produce 11 fractions. TLC analysis was then performed and the fractions with the same profiles were combined. The F1 fraction was separated by flash chromatography to produce 15 fractions from 113 vials. Fraction in vial number 90, 92, 94, 96 and 98 were combined and separated again by flash chromatography to produce 15 fractions from 102 vials. Fraction in vial number 43, 44, 45, 46, 47, 48, 49 and 50 were combined and coded as MO-M-V1-FL13a-FL8b compound. This compound was purified by recrystallization method and spectroscopic analysis was carried out using UV-Vis and FTIR spectroscopy. The results obtained were that there were no pure compounds found in the methanol extract of M. oleifera stem bark. UV-Vis spectrum analysis produced two absorption maximums at a wavelength (λmax) of 266 and 209 nm, so it can be seen that the MO-M-V1-FL13a-FL8b compound has conjugated double bonds and has free electrons. FTIR analysis showed that there were some bands at 3007 cm-1 (= C-H aromatic), 2925-2853 cm-1 (-C-H alkane), 1166-1075 cm-1 (-C-O) and 673 cm-1 (aromatic). The results of the toxicity test using the BSLT method showed that this extract had moderate toxicity to A. salina shrimp larvae with an LC50 value of 118,50 ppm.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1903113509

Penerbit

Pekanbaru : Universitas Riau - FKIP - Pendidikan Kimia., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1903113509

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

tethi

Versi lain/terkait

Lampiran Berkas

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