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Analisis Sekuens Barkode Dna Its Dan Rbcl Pada Tumbuhan Pepina (Pterococcus Corniculatus L.) Asal Riau

The pepina plant (Pterococcus corniculatus) is a vine and mellit plant from the family Euphorbiaceae. DNA barcoding is a technique to identify an organism using a short nucleotide sequence known as a DNA barcode. One of the DNA barcodes in plants is ITS and rbcL. The purpose of this research is to analyze the sequence of DNA barcodes in the ITS and rbcL regions of pepina plants, and confirm the scientific name of this plant by using the DNA barcode. Samples were taken from the area of Air Putih Village, Tuah Madani District, Pekanbaru, Riau Province, Indonesia as many as three different individuals. The research stages carried out are sampling, morphological observation, molecular analysis including DNA isolation using the Geneaid Mini Plant kit, total DNA electrophoresis, PCR, electrophoresis, sequencing, and data analysis using bioinformatics programs, namely BioEdit, BLASTn, and MEGA 6.0. The results of morphological observations showed that the pepina plant had a special characteristic, which is to have a vine, the shape of a star-shaped capsule fruit with 4 to 5 lobes. DNA barcoding analysis using ITS sequences succeeded in confirming pepina as Plukenetia corniculata. The results of the ITS DNA sequence analysis showed that P. corniculata had a 100% identity value with the pepina samples studied. Plukenetia corniculata has a synonym Pterococcus corniculatus which indicates that the pepina samples studied is correct as Pterococcus corniculatus. The results of the analysis of the rbcL DNA sequence showed that no identity value was found to reach 100%. The highest identity value was only 99.64%, namely Plukentia volubilis. The DNA sequence of rbcL pepina obtained in this study was the first to be reported to GenBank.

Key words: DNA barcode, ITS, pepina, Pterococcus corniculatus, rbcL.

Ketersediaan
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Perpustakaan Universitas Riau 2003113150
2003113150
Tersedia
Informasi Detail
Judul Seri
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No. Panggil
2003113150
Penerbit
Pekanbaru : Universitas Riau – FMIPA – Biologi.,
Deskripsi Fisik
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Bahasa
Indonesia
ISBN/ISSN
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Klasifikasi
2003113150
Tipe Isi
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Tipe Media
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Tipe Pembawa
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Edisi
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Subjek
BIOLOGI
Info Detail Spesifik
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Pernyataan Tanggungjawab
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