CD Tesis
Efek Pemberian Propolis Pada Larva Terhadap Rasio Sex Ikan Nila Merah (Oreochromis niloticus)
Red tilapia (Oreochromis niloticus) is potential to be cultivated in freshwater and seawater, as it is tolerant to environmental changes. Market demand for tilapia is increasing and this fish became a potential fisheries commodity. In the field of aquaculture, the growth of male fish is faster than the female, so the cultivation of red tilapia is directed more to the production of male fish. One way that can be applied to form male tilapia is with sex reversal techniques using propolis. Propolis is a natural ingredient that is safe and relatively cheap. It contains chrysin which is classified as a flavonoid that capable to inhibit the performance of the aromatase enzymes. Propolis can be given to red tilapia larvae by mixing it into the feed.
This study aims to analyse the effect of different doses of propolis administration on sex ratio and percentage of male red tilapia. This research was conducted for 90 days at the Laboratory of Aquaculture Technology, Department of Aquaculture, Faculty of Fisheries and Marine Science, Riau University. Result of this study will provide information of the right sex reversal method using propolis, including the optimal dose that can be applied to the community in the field.
The method applied in this study was a Completely Random Design, with 5 treatments and 3 replications. The propolis treatments applied in the study was the control treatment (no propolis/ P0), and 1.3 ml (P1); 2.0 ml (P2); 2.7 ml (P3) and 3.4ml (P4) of propolis in a kilo of fish feed pellet. The larvae used in the study were red tilapia larvae aged 5 days, with a body weight around 0.008 g. There were 20 fishes/container (20 L aquaria filled with freshwater and completed with an aerator). The fish was maintained for 90 days and fed with commercial fish feed pellets PF 0 (40-42% protein content ) and PF 100 (32 – 34% protein content) that was enriched with propolis, ad libitum (at satiation), 3 times/day. Parameters measured were sex ratio, percentage of males, absolute weight growth, absolute length growth, specific growth rate, survival and water quality.
Results shown that the provision of propolis with different doses affected the sex ratio and also triggered the larvae to develop into male fish. The highest proportion of male fish resulted from treatment P4 or 3.4 ml/kg (8.67 males: 1 female and 0.33 juveniles) or 86.67% males. In contrast, in the control, the sex ratio of fish was 6.67 males: 2.67 females and 0.67 juveniles. The best absolute weight growth was obtained from the 2.7 ml/kg treatment (P3), with fish weight reaching 4.22 grams. The best absolute length growth was obtained in the treatment without propolis (P0), with fish body length reaching 5.61. The best specific growth rate was obtained in the treatment of 2.7 ml/kg (P3) which was 7.94% and the best survival rate was obtained in the treatment of 2.7 ml/kg (P3) which was 98.33%. Water quality during the study was normal, namely: temperature 27.0-28.3 oC, pH 7.0-8.5, DO 5.9-6.7 mg/L, ammonia 0.02-0.06 mg/L, and nitrite 0.16-0.96 mg/l. The application of propolis triggers the red tilapia larvae to develop into male and propolis application also positively affects the absolute weight, absolute length, specific growth rate and survival rate.
Keywords: Red tilapia, larvae, sex reversal, propolis, chrysin.
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